High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption / ionisation time-of-flight mass spectrometry (MALDI-TOF MS) / P. Christ [et al.]
Уровень набора: European Journal of Mass Spectrometry , Scientific Journal = 1995-Язык: английский.Страна: .Резюме или реферат: Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus a very high, mass-independent, detection efficiency is expected from their application in TOF-MS. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series using standard sample preparation procedures shows that the detection limit for insulin (Mr: 5,734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF ß (Mr: 49,032) and for polyclonal IgG (Mr: ca. 150,000) in the high femtomole and low picomole range, respectively, were established.Примечания о наличии в документе библиографии/указателя: [Ref.: (23 tit.)].Тематика: электронный ресурс | труды учёных ТПУ | криогенные детекторы | cryogenic detector | масс-спектрометрия | MALDI Ресурсы он-лайн:Щелкните здесь для доступа в онлайн | Щелкните здесь для доступа в онлайнTitle screen
[Ref.: (23 tit.)]
Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus a very high, mass-independent, detection efficiency is expected from their application in TOF-MS. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series using standard sample preparation procedures shows that the detection limit for insulin (Mr: 5,734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF ß (Mr: 49,032) and for polyclonal IgG (Mr: ca. 150,000) in the high femtomole and low picomole range, respectively, were established
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