“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(l-lactic acid) electrospun scaffolds / S. I. Goreninsky, R. O. Gulyaev, K. S. Stankevich [et al.]
Уровень набора: Colloids and Surfaces B: BiointerfacesЯзык: английский.Резюме или реферат: In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0°, enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07?±?0.01?mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength..Примечания о наличии в документе библиографии/указателя: [References: 19 tit.].Аудитория: .Тематика: электронный ресурс | труды учёных ТПУ | polylactic acid | gelatin | electrospun scaffolds | surface modification | желатин | поверхностный слой | строительные леса Ресурсы он-лайн:Щелкните здесь для доступа в онлайнTitle screen
[References: 19 tit.]
In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0°, enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07?±?0.01?mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
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