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Reflection photonic jet (r-PJ) is a new type of photonic jet (PJ), formed in reflection mode, which has the potential to revolutionize mesoscale photonics in many applications, e.g. optical nanoparticle trapping, liquids and gas refractive index sensing, signal switching, etc. In this paper, we present a microfluidic channel based on a hollow micro-fiber structure with partially coated optically thick gold film to produce r-PJs for biofluid application. The proposed scheme is studied by the full-wave simulations using the finite element method. The key r-PJ parameters, such as the maximum field intensity, focus position and full width at half-maximum (FWHM) are studied for different size of the microchannel and coated area. The proposed design allows a wide range of flexible control of the r-PJ parameters and can be easily integrated into microchannel systems.