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071	6	0	_a10.1152/ajpheart.00460.2011
090			_a639061
100			_a20150219a2011 k y0engy50 ba
101	0		_aeng
102			_aUS
135			_adrcn ---uucaa
181		0	_ai
182		0	_ab
200	1		_aDo TRPC-like currents and G protein-coupled receptors interact to facilitate myogenic tone development? _fYa. J. Anfinogenova [et al.]
203			_aText _celectronic
300			_aTitle screen
320			_a[References: 43 tit.]
330			_aThe objective of this study was to determine whether Gq/11-coupled receptor activation can enhance the mechanosensitivity of a canonical transient receptor potential (TRPC)-like current and consequently the myogenic responsiveness of rat anterior cerebral arteries. Initial patch-clamp experiments revealed the presence of a basal cation current in isolated smooth muscle cells that displayed evidence of double rectification, which was blocked by trivalent cations (Gd3+ and La3+). PCR analysis identified the expression of TRPC1, 3, 6 and 7 mRNA and, characteristic of TRPC-like current, the whole-cell conductance was insensitive to a Na+-dependent transport (amiloride), TRP vanilloid (ruthenium red), and chloride channel (DIDS, niflumic acid, and flufenamate) inhibitors. One notable exception was tamoxifen, which elicited a dual effect, blocking or activating the TRPC-like current at 1 and 10 ?M, respectively. This TRPC-like current was augmented by constrictor agonists (uridine 5?-triphosphate and U46619) or hyposmotic challenge (303 to 223 mOsm/l), a mechanical stimulus. Although each stimulus was effective alone, smooth muscle cells pretreated with agonist did not augment the whole-cell response to hyposmotic challenge. Consistent with these electrophysiological recordings, functional experiments revealed that neither UTP nor U46619 enhanced the sensitivity of intact cerebral arteries to hyposmotic challenge or elevated intravascular pressure. In summary, this study found no evidence that Gq/11-coupled receptor activation augments the mechanosensitivity of a TRPC-like current and consequently the myogenic responsiveness of anterior cerebral arteries.
333			_aРежим доступа: по договору с организацией-держателем ресурса
461			_tAmerican Journal of Physiology - Heart and Circulatory Physiology _oScientific Journal _d1997-
463			_tVol. 301, № 4 _v[11 p.] _d2011
610	1		_aэлектронный ресурс
610	1		_aтруды учёных ТПУ
701		1	_aAnfinogenova _bYa. J. _cmedic _cLecturer of Tomsk Polytechnic University, Doctor of medical sciences _f1970- _gYana Jonovna _2stltpush _3(RuTPU)RU\TPU\pers\33592
701		1	_aBrett _bS. E.
701		1	_aWalsh _bM. P.
701		1	_aHarraz _bO. F.
701		1	_aWelsh _bD. G.
801		2	_aRU _b63413507 _c20150317 _gRCR
856	4		_uhttp://ajpheart.physiology.org/content/301/4/H1378.full-text.pdf+html
942			_cCF