| 000 | 03577nla2a2200409 4500 | ||
|---|---|---|---|
| 001 | 640462 | ||
| 005 | 20231030040356.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\5011 | ||
| 035 | _aRU\TPU\network\5007 | ||
| 090 | _a640462 | ||
| 100 | _a20150417a2015 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 105 | _ay z 100zy | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aNew Porphyrins/Calf Thymus DNA Complexes - Their Thermostability _fL. Aloyan, Y. Dalyan, A. S. Gogolev |
|
| 203 |
_aText _celectronic |
||
| 225 | 1 | _aRadiation Technologies in Medicine | |
| 300 | _aTitle screen | ||
| 330 | _aThe paper studies the melting parameters of the complexes of water soluble cationic 3N- and 4N-pyridyl porphyrins with different peripheral substituents (oxyethyl, buthyl, allyl, metallyl) with DNA. The results indicate that the presence of porphyrin changes the shape and parameters of DNA melting curve. The porphyrin concentration increase results in the increase of the melting temperature (Tm) and melting interval (deltaT) of DNA. With the porphyrin-DNA concentration ratio v=0.01, changes of melting temperature have not been observed. The melting intervals almost do not change upon the addition of the 4N- porphyrins, while the decrease of deltaT is observed in the presence of 3N-porphyrins. Because of the intercalation binding mechanism occurring in GC-rich regions of DNA, we assume that 3N-porphyrins intercalated in GC-rich regions, reduce the thermal stability of these sites, bringing them closer to the thermal stability of the AT-sites, which is the reason for the decrease in melting interval. While with the relative concentration v=0.01 for 4-N porphyrins the external binding mechanism "turns on" and destabilizing effect of porphyrins on GC-pairs compensated by their stabilizing effect on AT-pairs. As a result, no change in the melting parameters of DNA is observed upon complexation with these porphyrins. | ||
| 333 | _aРежим доступа: по договору с организацией-держателем ресурса | ||
| 461 | 1 |
_0(RuTPU)RU\TPU\network\4598 _tAdvanced Materials Research : Radiation and nuclear techniques in material science _oScientific Journal |
|
| 463 | 1 |
_0(RuTPU)RU\TPU\network\4600 _tVol. 1084 : Physical-Technical Problems of Nuclear Science, Energy Generation, and Power Industry (PTPAI -2014) _oThe VIth International Conference, June 5-7, 2014, Tomsk, Russia _o[proceedings] _fNational Research Tomsk Polytechnic University (TPU) _v[P. 554-558] _d2015 |
|
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aДНК | |
| 610 | 1 | _aпорфирины | |
| 610 | 1 | _aплавление | |
| 610 | 1 | _aтермостабильность | |
| 700 | 1 |
_aAloyan _bL. _cphysicist _cresearcher of Tomsk Polytechnic University, candidate of physico-mathematical Sciences _f1979- _gLusine _2stltpush _3(RuTPU)RU\TPU\pers\39848 |
|
| 701 | 1 |
_aDalyan _bY. |
|
| 701 | 1 |
_aGogolev _bA. S. _cphysicist _cassociate professor of Tomsk Polytechnic University _f1983- _gAleksey Sergeevich _2stltpush _3(RuTPU)RU\TPU\pers\31537 |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет (ТПУ) _bФизико-технический институт (ФТИ) _bКафедра прикладной физики (№ 12) (ПФ) _h46 _2stltpush _3(RuTPU)RU\TPU\col\18729 |
| 801 | 2 |
_aRU _b63413507 _c20171122 _gRCR |
|
| 856 | 4 | _uhttp://dx.doi.org/10.4028/www.scientific.net/AMR.1084.554 | |
| 942 | _cCF | ||