| 000 | 03509nla2a2200481 4500 | ||
|---|---|---|---|
| 001 | 641252 | ||
| 005 | 20231030040422.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\6152 | ||
| 035 | _aRU\TPU\network\6151 | ||
| 090 | _a641252 | ||
| 100 | _a20150514a2015 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aUS | ||
| 105 | _ay z 100zy | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aDevelopment of a Method for Producing and Purifying N2-Carboxyethyl-2'-Deoxyguanosine for Molecular-Biological Research _fS. V. Krivoshchekov [et al.] |
|
| 203 |
_aText _celectronic |
||
| 225 | 1 | _aMaterial Science in Biology and Medicine | |
| 300 | _aTitle screen | ||
| 330 | _aMethylglyoxal (MG) – is a high reactive α- oxoaldehyde, which can be synthesized through various biochemical processes in vivo. MG is capable of interacting with nucleophilic groups of proteins, lipids and nucleic acids which leads to their glycation. Covalent compounds that formed in this process were named advanced glycation end products. Advanced glycation end products play an important role in the pathogenesis of diabetes, chronic inflammation, cancer and Alzheimer's disease. One of the most stable compounds, which is formed by reacting of methylglyoxal with the nitrogenous bases of the DNA molecule is N2- carboxyethyl -2' - deoxyguanosine (CEdG). This compound can be used as a marker for monitoring of various diseases, investigation of the role of glycation end products in their pathogenesis, as well as to search for the therapeutic targets. To perform our molecular-biological investigations by mass spectrometry, we synthesized N2- carboxyethyl -2' - deoxyguanosine, and have proposed a new method for purifying compounds by reverse - phase HPLC. As a result, we achieved the optimal purity of the sample, while substantially reducing the cost of the purification procedure. | ||
| 333 | _aРежим доступа: по договору с организацией-держателем ресурса | ||
| 461 | 0 |
_0(RuTPU)RU\TPU\network\4598 _tAdvanced Materials Research _oScientific Journal |
|
| 463 | 0 |
_0(RuTPU)RU\TPU\network\4656 _tVol. 1085 : Prospects of Fundamental Sciences Development (PFSD-2014) _oThe XIth International Conference, April 22-25, 2014, Tomsk, Russia _o[proceedings] _v[P. 436-440] _d2015 |
|
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aмасс-спектрометрия | |
| 610 | 1 | _aметилглиоксаль | |
| 610 | 1 | _aбиологические исследования | |
| 701 | 1 |
_aKrivoshchekov _bS. V. _cchemist _cengineer of Tomsk Polytechnic University _f1987- _gSergey Vladimirovich _2stltpush _3(RuTPU)RU\TPU\pers\34574 |
|
| 701 | 1 |
_aBogdanov _bA. O. |
|
| 701 | 1 |
_aOgorodova _bL. M. |
|
| 701 | 1 |
_aDementeva _bN. B. |
|
| 701 | 1 |
_aSaltykova _bI. V. |
|
| 701 | 1 |
_aMarchenko _bR. D. |
|
| 701 | 1 |
_aIvanov _bV. V. |
|
| 701 | 1 |
_aMayboroda _bO. A. |
|
| 701 | 1 |
_aSazonov _bA. E. |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет (ТПУ) _bИнститут природных ресурсов (ИПР) _bКафедра технологии органических веществ и полимерных материалов (ТОВПМ) _h6832 _2stltpush _3(RuTPU)RU\TPU\col\18659 |
| 801 | 2 |
_aRU _b63413507 _c20161229 _gRCR |
|
| 856 | 4 | _uhttp://dx.doi.org/10.4028/www.scientific.net/AMR.1085.436 | |
| 942 | _cCF | ||