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101 0 _aeng
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181 0 _ai
182 0 _ab
200 1 _a3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus
_fA. M. Demin [et al.]
203 _aText
_celectronic
300 _aTitle screen
320 _a[References: p. 4462-4463 (55 tit.)]
330 _aPurpose: Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites. Materials and methods: Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe3+ and Fe2+ salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis . Magnetic resonance properties of MNPs were investigated in vitro and in vivo. Results: The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g-1) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r2 was 271 mmol-1 s-1. To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus. Conclusion: The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage.
461 _tInternational Journal of Nanomedicine
_d2006-
463 _tVol. 11
_v[P. 4451-4463]
_d2016
610 1 _aтруды учёных ТПУ
610 1 _aэлектронный ресурс
610 1 _aмагнитные наночастицы
610 1 _aалкоксисиланы
610 1 _aмагнитно-резонансная томография
610 1 _aпечень
701 1 _aDemin
_bA. M.
_gAlexander Mikhaylovich
701 1 _aPershina
_bA. G.
_cbiologist
_cAssociate Professor of Tomsk Polytechnic University, Candidate of biological sciences
_f1981-
_gAleksandra Gennadievna
_2stltpush
_3(RuTPU)RU\TPU\pers\32466
701 1 _aIvanov
_bV. V.
_gVladimir Vladimirovich
701 1 _aNevskaya
_bK. V.
_gKseniya Vladimirovna
701 1 _aShevelev
_bO. B.
_gOleg Borisovich
701 1 _aMinin
_bA. S.
_gArtyom Sergeevich
701 1 _aByzov
_bI. V.
_gIliya Vladimrovich
701 1 _aSazonov
_bA. E.
_gAlexey Eduardovich
701 1 _aKrasnov
_bV. P.
_gVictor Pavlovich
701 1 _aOgorodova
_bL. M.
_gLudmila Mikhaylovna
712 0 2 _aНациональный исследовательский Томский политехнический университет (ТПУ)
_bИнститут физики высоких технологий (ИФВТ)
_bКафедра биотехнологии и органической химии (БИОХ)
_h6810
_2stltpush
_3(RuTPU)RU\TPU\col\18693
801 2 _aRU
_b63413507
_c20170809
_gRCR
856 4 0 _uhttp://dx.doi.org/10.2147/IJN.S111880
942 _cCF