| 000 | 04139nlm1a2200481 4500 | ||
|---|---|---|---|
| 001 | 655215 | ||
| 005 | 20231030041335.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\21126 | ||
| 035 | _aRU\TPU\network\21125 | ||
| 090 | _a655215 | ||
| 100 | _a20170809a2016 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aNZ | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_a3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus _fA. M. Demin [et al.] |
|
| 203 |
_aText _celectronic |
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| 300 | _aTitle screen | ||
| 320 | _a[References: p. 4462-4463 (55 tit.)] | ||
| 330 | _aPurpose: Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites. Materials and methods: Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe3+ and Fe2+ salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis . Magnetic resonance properties of MNPs were investigated in vitro and in vivo. Results: The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g-1) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r2 was 271 mmol-1 s-1. To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus. Conclusion: The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage. | ||
| 461 |
_tInternational Journal of Nanomedicine _d2006- |
||
| 463 |
_tVol. 11 _v[P. 4451-4463] _d2016 |
||
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aмагнитные наночастицы | |
| 610 | 1 | _aалкоксисиланы | |
| 610 | 1 | _aмагнитно-резонансная томография | |
| 610 | 1 | _aпечень | |
| 701 | 1 |
_aDemin _bA. M. _gAlexander Mikhaylovich |
|
| 701 | 1 |
_aPershina _bA. G. _cbiologist _cAssociate Professor of Tomsk Polytechnic University, Candidate of biological sciences _f1981- _gAleksandra Gennadievna _2stltpush _3(RuTPU)RU\TPU\pers\32466 |
|
| 701 | 1 |
_aIvanov _bV. V. _gVladimir Vladimirovich |
|
| 701 | 1 |
_aNevskaya _bK. V. _gKseniya Vladimirovna |
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| 701 | 1 |
_aShevelev _bO. B. _gOleg Borisovich |
|
| 701 | 1 |
_aMinin _bA. S. _gArtyom Sergeevich |
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| 701 | 1 |
_aByzov _bI. V. _gIliya Vladimrovich |
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| 701 | 1 |
_aSazonov _bA. E. _gAlexey Eduardovich |
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| 701 | 1 |
_aKrasnov _bV. P. _gVictor Pavlovich |
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| 701 | 1 |
_aOgorodova _bL. M. _gLudmila Mikhaylovna |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет (ТПУ) _bИнститут физики высоких технологий (ИФВТ) _bКафедра биотехнологии и органической химии (БИОХ) _h6810 _2stltpush _3(RuTPU)RU\TPU\col\18693 |
| 801 | 2 |
_aRU _b63413507 _c20170809 _gRCR |
|
| 856 | 4 | 0 | _uhttp://dx.doi.org/10.2147/IJN.S111880 |
| 942 | _cCF | ||