| 000 | 02826nlm1a2200433 4500 | ||
|---|---|---|---|
| 001 | 655503 | ||
| 005 | 20231030041347.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\21632 | ||
| 035 | _aRU\TPU\network\21575 | ||
| 090 | _a655503 | ||
| 100 | _a20170906a2017 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aNL | ||
| 135 | _adrnn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aSpheroids of HER2-Positive Breast Adenocarcinoma for Studying Anticancer Immunotoxins In Vitro _fI. V. Balalaeva [et al.] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: p. 43 (32 tit.)] | ||
| 330 | _aTumor response to therapeutic treatment is largely determined by its heterogeneity and the presence of intercellular junctions, hindering the penetration of large molecules deep into the three-dimensional structure of the tumor. In that context, 3D in vitro tumor models such as cancer cell spheroids are becoming increasingly popular. We obtained spheroids of human breast adenocarcinoma SKBR-3 overexpressing the HER2 cancer marker. The toxicity of HER2-targeted immunotoxin 4D5scFv-PE40 against spheroids was shown to be several orders of magnitude lower compared to a monolayer cell culture. The significant difference in the severity of the immunotoxin effect can be explained by the fact that it ineffectively penetrates the spheroid and predominantly influences the cells of the outer layers. The resulting tumor spheroid model can be used in development of drugs for targeted therapy as well as to study ways to improve the efficiency of anticancer agents by targeting cell-cell contacts. | ||
| 461 | _tActa Naturae | ||
| 463 |
_tVol. 9, №1 (32) _v[P. 38-43] _d2017 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aопухоли | |
| 610 | 1 | _aсфероиды | |
| 610 | 1 | _a3D-модели | |
| 610 | 1 | _aсфероиды | |
| 610 | 1 | _aсоединения | |
| 701 | 1 |
_aBalalaeva _bI. V. _gIrina Vladimirovna |
|
| 701 | 1 |
_aSokolova _bE. A. _gEvgeniya Anatoljevna |
|
| 701 | 1 |
_aPuzhikhina _bA. D. _gAlena Dmitrievna |
|
| 701 | 1 |
_aBrilkina _bA. A. _gAnna Aleksandrovna |
|
| 701 | 1 |
_aDeev _bS. M. _cbiologist _cthe expert of Tomsk Polytechnic University, doctor of biological Sciences _gSergey Mikhaylovich _2stltpush _3(RuTPU)RU\TPU\pers\39299 |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет (ТПУ) _bФизико-технический институт (ФТИ) _bЛаборатория радиационного контроля № 31 (Лаборатория РК № 31) _h6472 _2stltpush _3(RuTPU)RU\TPU\col\19161 |
| 801 | 2 |
_aRU _b63413507 _c20170906 _gRCR |
|
| 856 | 4 | _uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406658/ | |
| 942 | _cCF | ||