| 000 | 03107nlm1a2200457 4500 | ||
|---|---|---|---|
| 001 | 656696 | ||
| 005 | 20231030041440.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\23162 | ||
| 090 | _a656696 | ||
| 100 | _a20171207a2017 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aNL | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aDeep-penetrating photodynamic therapy with KillerRed mediated by upconversion nanoparticles _fLiuen Liang [et al.] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: 45 tit.] | ||
| 330 | _aThe fluorescent protein KillerRed, a new type of biological photosensitizer, is considered as a promising substitute for current synthetic photosensitizes used in photodynamic therapy (PDT). However, broad application of this photosensitiser in treating deep-seated lesions is challenging due to the limited tissue penetration of the excitation light with the wavelength falling in the visible spectral range. To overcome this challenge, we employ upconversion nanoparticles (UCNPs) that are able to convert deep-penetrating near infrared (NIR) light to green light to excite KillerRed locally, followed by the generation of reactive oxygen species (ROS) to kill tumour cells under centimetre-thick tissue. The photosensitizing bio-nanohybrids, KillerRed-UCNPs, are fabricated through covalent conjugation of KillerRed and UCNPs. The resulting KillerRed-UCNPs exhibit excellent colloidal stability in biological buffers and low cytotoxicity in the dark. Cross-comparison between the conventional KillerRed and UCNP-mediated KillerRed PDT demonstrated superiority of KillerRed-UCNPs photosensitizing by NIR irradiation, manifested by the fact that ?70% PDT efficacy was achieved at 1-cm tissue depth, whereas that of the conventional KillerRed dropped to ~7%. | ||
| 461 | _tActa Biomaterialia | ||
| 463 |
_tVol. 51 _v[P. 461-470] _d2017 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aфотодинамическая терапия | |
| 610 | 1 | _aфотосенсибилизаторы | |
| 610 | 1 | _aбелок | |
| 610 | 1 | _aнаночастицы | |
| 610 | 1 | _aпередача энергии | |
| 701 | 0 | _aLiuen Liang | |
| 701 | 0 | _aYiqing Lu | |
| 701 | 0 | _aRun Zhang | |
| 701 | 1 |
_aCare _bA. _gAndrew |
|
| 701 | 1 |
_aOrtega _bT. A. _gTiago Almeida |
|
| 701 | 1 |
_aDeev _bS. M. _cbiologist _cthe expert of Tomsk Polytechnic University, doctor of biological Sciences _gSergey Mikhaylovich _2stltpush _3(RuTPU)RU\TPU\pers\39299 |
|
| 701 | 1 |
_aQian _bYi. |
|
| 701 | 1 |
_aZvyagin _bA. V. _gAndrey V. |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет (ТПУ) _bФизико-технический институт (ФТИ) _bЛаборатория радиационного контроля № 31 (Лаборатория РК № 31) _h6472 _2stltpush _3(RuTPU)RU\TPU\col\19161 |
| 801 | 2 |
_aRU _b63413507 _c20171220 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.1016/j.actbio.2017.01.004 | |
| 942 | _cCF | ||