| 000 | 03455nlm1a2200445 4500 | ||
|---|---|---|---|
| 001 | 660150 | ||
| 005 | 20231030041655.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\29118 | ||
| 090 | _a660150 | ||
| 100 | _a20190429a2018 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aIn vitro effects of a novel silver-based complex on influenza virus _fV. A. Silnikov, E. V. Plotnikov |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: 26 tit.] | ||
| 330 | _aObjective: To study the mechanism of antiviral activity of a new silver-based compound of general formula C6H19Ag2N4 LiO6S2 on influenza virus. Materials and Methods: Antiviral properties were investigated on test culture of Madin–Darby canine kidney (MDCK) cells with serial tenfold dilutions of the influenza virus. The completeness of viral inactivation was confirmed by determining the virus titer after a double passage of virus-containing suspension in MDCK cell culture. Reverse transcription polymerase chain reaction analysis of viral RNA, enzyme immunoassay, and transmission electron microscopy for morphological analysis of influenza virus particles were applied for analysis of viral RNA and effect of substance influence. Results: The results indicate a significant change in the antigenic structure of viral proteins of influenza virus by the silver-containing complex. The main inactivation mechanism of influenza virus by the action of the silver-containing complex is associated primarily with the effect on virion protein structure rather than cleavage of the viral RNA. It was revealed that the mechanism for the inactivation of influenza virus is not associated with cleavage of viral RNA corresponding genes M, NS, or nucleoprotein but more probable with changes of the antigen determinants of virion. It was found a significant damaging effect on influenza virus under action of tested substance at concentration higher than 0.4 mM. Conclusion: The novel silver-based complex possesses antiviral properties and could be considered as a prospective antiviral drug. | ||
| 461 | _tJournal of Pharmacology and Pharmacotherapeutics | ||
| 463 |
_tVol. 4, iss. 9 _v[P. 186-190] _d2018 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aantiviral activity | |
| 610 | 1 | _ainfluenza virus | |
| 610 | 1 | _amonoclonal antibodies | |
| 610 | 1 | _apolymerase chain reaction | |
| 610 | 1 | _asilver-based complex | |
| 610 | 1 | _aпротивовирусные препараты | |
| 610 | 1 | _aвирусы | |
| 610 | 1 | _aгрипп | |
| 610 | 1 | _aмоноклональные антитела | |
| 610 | 1 | _aполимеразная цепная реакция | |
| 700 | 1 |
_aSilnikov _bV. A. _gVladimir |
|
| 701 | 1 |
_aPlotnikov _bE. V. _cchemist _cEngineer of Tomsk Polytechnic University _f1983- _gEvgeny Vladimirovich _2stltpush _3(RuTPU)RU\TPU\pers\32469 |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий (ИШХБМТ) _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20200130 _gRCR |
|
| 856 | 4 | _uhttp://earchive.tpu.ru/handle/11683/57714 | |
| 856 | 4 | _uhttp://dx.doi.org/10.4103/jpp.JPP_50_18 | |
| 942 | _cCF | ||