| 000 | 03727nlm1a2200421 4500 | ||
|---|---|---|---|
| 001 | 661954 | ||
| 005 | 20231030041804.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\33046 | ||
| 035 | _aRU\TPU\network\29958 | ||
| 090 | _a661954 | ||
| 100 | _a20200324a2020 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aLethal effects of silver nanoparticles on Perkinsus marinus, a protozoan oyster parasite _fC. Bravo-Guerra, J. Caceres-Martinez, R. Vasquez-Yeomans [et al.] |
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| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 330 | _aPerkinsus marinus, a World Organisation for Animal Health (OIE) notifiable parasite, infects several species of oyster, including Crassostrea virginica and Crassostrea corteziensis. There is little information on possible treatments for this parasite, but the biocidal properties of silver nanoparticles (AgNP) suggest their potential use. The lethal effects of the Argovit™ formulation of AgNP was evaluated for the first time against hypnospores of P. marinus, a particularly resistant stage of the parasite that persists in the environment until favorable conditions occur for zoosporulation to be induced. Hypnospores were exposed to 1, 10 and 100 µg/mL of silver compounded in Argovit™ (corresponding to 0.009, 0.093 and 0.927 mM of Ag), to 157.47 µg/mL (0.927 mM) of silver nitrate (AgNO3) used as a positive control, and to polyvinylpyrrolidone (PVP, 1570 µg/mL) used as a vehicle control. Hypnospores in culture medium without treatment served as a negative control. Dose-dependence after 24 h of exposure to AgNP was observed. A concentration of 0.093 mM AgNP resulted in 50% mortality of P. marinus. Treatment with 0.927 mM of silver, as AgNP or AgNO3, was highly lethal, with greater than 90% mortality. Silver nanoparticles were implicated in the deformation of hypnospores. Transmission electron microscopy (TEM) revealed AgNP within the hypnospore wall and involved in the degradation of lipid droplets in the cytoplasm. AgNP were effective in a saline medium, suggesting the utility of detailed studies of the physicochemical interactions of AgNP under these conditions. These results suggest investigations of possible effect of Argovit™ formulation of AgNP against stages of the parasite like trophozoites and tomonts that develop in tissues or hemolymph of infected oysters as well as studies on its effects in the host and environment. | ||
| 333 | _aРежим доступа: по договору с организацией-держателем ресурса | ||
| 461 | _tJournal of Invertebrate Pathology | ||
| 463 |
_tVol. 169 _v[107304, 5 p.] _d2020 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _ahypnospores | |
| 610 | 1 | _aperkinsus marinus | |
| 610 | 1 | _asilver nanoparticles | |
| 610 | 1 | _aнаночастицы | |
| 610 | 1 | _aсеребро | |
| 701 | 1 |
_aBravo-Guerra _bC. _gCecilia |
|
| 701 | 1 |
_aCaceres-Martinez _bJ. _gJorge |
|
| 701 | 1 |
_aVasquez-Yeomans _bR. _gRebeca |
|
| 701 | 1 |
_aPestryakov _bA. N. _cChemist _cProfessor of Tomsk Polytechnic University, Doctor of Chemical Science _f1963- _gAleksey Nikolaevich _2stltpush _3(RuTPU)RU\TPU\pers\30471 |
|
| 701 | 1 |
_aBogdanchikova _bN. _gNina |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий (ИШХБМТ) _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20200324 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.1016/j.jip.2019.107304 | |
| 942 | _cCF | ||