| 000 | 03511nlm1a2200493 4500 | ||
|---|---|---|---|
| 001 | 664190 | ||
| 005 | 20231030041921.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\35374 | ||
| 035 | _aRU\TPU\network\35018 | ||
| 090 | _a664190 | ||
| 100 | _a20210331a2021 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aCH | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aRegeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Buds _fC. Pereira, I. A. Montalban, A. Pedrosa [et al.] |
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| 203 |
_aText _celectronic |
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| 300 | _aTitle screen | ||
| 320 | _a[References: 55 tit.] | ||
| 330 | _aOrganogenesis and somatic embryogenesis have been widely applied as the two main regeneration pathways in plant tissue cultures. However, recalcitrance is still the main restriction in the clonal propagation of many woody species, especially in conifers. They undergo a “phase change” that leads to significant loss of vegetative propagation capacity, reducing the aptitude of tissues and organs to be regenerated in vitro beyond this point. In line with this, the in vitro regeneration of mature conifer trees has been a long-cherished goal in many laboratories worldwide. Based on previous works in Pinus species regeneration from adult trees, we now present data about the culture of apical shoot buds in an attempt to induce organogenesis and somatic embryogenesis to clone mature trees of Aleppo pine (Pinus halepensis). Reinvigorated axillary shoots were submitted to conditions usually applied to induce somatic embryogenesis through the manipulation of culture media, including the use of auxins such as 2,4-Dichlorophenoxyacetic acid and 1-Naphthaleneacetic acid, cytokinins (6-benzyladenine and kinetin), and phytosulfokine (50, 100, and 200 nM). Although somatic embryos could not be obtained, an embryogenic-like tissue was produced, followed by the emergence of actively proliferating non-embryogenic calli. Variations in the consistence, texture, and color of non-embryogenic calli were observed; especially those arising in the media containing phytosulfokine. Reinvigorated shoots, induced by 22 or 44 µM 6-benzyladenine, were obtained through organogenesis and acclimatized, and phenotypically normal plants were obtained. | ||
| 461 | _tForests | ||
| 463 |
_tVol. 12, iss. 3 _v[363, 15 p.] _d2021 |
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| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aAleppo pine | |
| 610 | 1 | _aconifers | |
| 610 | 1 | _aphytosulfokine | |
| 610 | 1 | _aplant growth regulators | |
| 610 | 1 | _arooting | |
| 610 | 1 | _aсосны | |
| 610 | 1 | _aхвойные породы | |
| 701 | 1 |
_aPereira _bC. _gCatia |
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| 701 | 1 |
_aMontalban _bI. A. _gItziar |
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| 701 | 1 |
_aPedrosa _bA. _gAna |
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| 701 | 1 |
_aTavares _bJ. _gJessica |
|
| 701 | 1 |
_aPestryakov _bA. N. _cChemist _cProfessor of Tomsk Polytechnic University, Doctor of Chemical Science _f1963- _gAleksey Nikolaevich _2stltpush _3(RuTPU)RU\TPU\pers\30471 |
|
| 701 | 1 |
_aBogdanchikova _bN. _gNina |
|
| 701 | 1 |
_aCanhoto _bJ. _gJorge |
|
| 701 | 1 |
_aMoncalean _bP. _gPaloma |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20210331 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.3390/f12030363 | |
| 942 | _cCF | ||