| 000 | 03234nlm1a2200481 4500 | ||
|---|---|---|---|
| 001 | 664360 | ||
| 005 | 20231030041927.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\35544 | ||
| 035 | _aRU\TPU\network\20714 | ||
| 090 | _a664360 | ||
| 100 | _a20210412a2021 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aLayer-by-Layer technique as a versatile tool for gene delivery applications _fD. S. Linnik, Ya. V. Tarakanchikova, M. V. Zyuzin [et al.] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 330 | _aIntroduction: Gene therapy is a breakthrough medical field which focuses on the therapeutic delivery of recombinant nucleic acids in order to treat or prevent a broad spectrum of diseases. However, a number of important obstacles remain before its wide introduction into clinical practice can be envisaged. One of the biggest bottlenecks is the lack of efficient and safe delivery technologies, particularly, for in vivo distribution. Above and beyond standard requirements for carriers, the delivery systems for gene therapy ideally use a hit-and-run principle (to minimize off-target effect and display of immunogenic moieties). None of the currently used viral vectors fulfills all of these requirements. Therefore, the growing variety of non-viral delivery platforms represents a promising alternative. Areas covered: This review summarizes the Layer-by-Layer (LbL) approaches that can be effectively used for the gene delivery, considering various examples with the transfer of pDNA, mRNA, siRNA as well as genome-editing tools. Ex vivo gene modification of clinically relevant cells and clinical aspects for possible application of LbL systems in gene therapy are also underlined. Expert opinion: The LbL technique provides broad opportunities for the delivery of genetic material for various purposes and offers promise for future clinical application in gene therapy. | ||
| 461 | _tExpert Opinion on Drug Delivery | ||
| 463 |
_tVol. XX, iss. X _v[P. 1-19] _d2021 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _alayer-by-layer | |
| 610 | 1 | _atemplates | |
| 610 | 1 | _amultilayer capsules | |
| 610 | 1 | _agene delivery | |
| 610 | 1 | _aRNA | |
| 610 | 1 | _aDNA | |
| 610 | 1 | _aex vivo cell modification | |
| 610 | 1 | _aclinical perspective | |
| 610 | 1 | _aслои | |
| 701 | 1 |
_aLinnik _bD. S. _gDmitrii Sergeevich |
|
| 701 | 1 |
_aTarakanchikova _bYa. V. _gYana Vladimirovna |
|
| 701 | 1 |
_aZyuzin _bM. V. _gMikhail |
|
| 701 | 1 |
_aLepik _bK. V. |
|
| 701 | 1 |
_aAerts _bJ. L. _gJoeri |
|
| 701 | 1 |
_aSukhorukov _bG. _gGleb |
|
| 701 | 1 |
_aTimin _bA. S. _cChemist _cAssociate Scientist of Tomsk Polytechnic University _f1989- _gAleksandr Sergeevich _2stltpush _3(RuTPU)RU\TPU\pers\37036 |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20210412 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.1080/17425247.2021.1879790 | |
| 942 | _cCF | ||