| 000 | 03919nlm1a2200589 4500 | ||
|---|---|---|---|
| 001 | 664517 | ||
| 005 | 20231030041932.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\35701 | ||
| 035 | _aRU\TPU\network\28422 | ||
| 090 | _a664517 | ||
| 100 | _a20210416a2018 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aCH | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aExposure to Silver Nanospheres Leads to Altered Respiratory Mechanics and Delayed Immune Response in an in Vivo Murine Model _fDanielle Botelho, B. F. Leo, Christopher Massa [et al.] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: 21 tit.] | ||
| 330 | _aHere we examine the organ level toxicology of both carbon black (CB) and silver nanoparticles (AgNP). We aim to determine metal-specific effects to respiratory function, inflammation and potential interactions with lung lining fluid (LLF). C57Bl6/J male mice were intratracheally instilled with saline (control), low (0.05 [mu]g/g) or high (0.5 [mu]g/g) doses of either AgNP or CB 15 nm nanospheres. Lung histology, cytology, surfactant composition and function, inflammatory gene expression, and pulmonary function were measured at 1, 3, and 7 days post-exposure. Acutely, high dose CB resulted in an inflammatory response, increased neutrophilia and cytokine production, without alteration in surfactant composition or respiratory mechanics. Low dose CB had no effect. Neither low nor high dose AgNPs resulted in an acute inflammatory response, but there was an increase in work of breathing. Three days post-exposure with CB, a persistent neutrophilia was noted. High dose AgNP resulted in an elevated number of macrophages and invasion of lymphocytes. Additionally, AgNP treated mice displayed increased expression of IL1B, IL6, CCL2, and IL10. However, there were no significant changes in respiratory mechanics. At day 7, inflammation had resolved in AgNP-treated mice, but tissue stiffness and resistance were significantly decreased, which was accompanied by an increase in surfactant protein D (SP-D) content. These data demonstrate that the presence of metal alters the response of the lung to nanoparticle exposure. AgNP-surfactant interactions may alter respiratory function and result in a delayed immune response, potentially due to modified airway epithelial cell function. | ||
| 461 | _tFrontiers in Pharmacology | ||
| 463 |
_tVol. 9 _v[213, 9 p.] _d2018 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _asilver nanoparticles | |
| 610 | 1 | _alung | |
| 610 | 1 | _apulmonary function | |
| 610 | 1 | _asurfactant | |
| 610 | 1 | _ainflammation | |
| 610 | 1 | _aнаночастицы | |
| 610 | 1 | _aлегкие | |
| 610 | 1 | _aсеребро | |
| 610 | 1 | _aвоспаление | |
| 610 | 1 | _aиммунные процессы | |
| 701 | 1 |
_aBotelho _bDanielle _gD. |
|
| 701 | 1 |
_aLeo _bB. F. _gBey Fen |
|
| 701 | 1 |
_aMassa _bChristopher _gCh. B. |
|
| 701 | 1 |
_aSarkar _bS. _gSrijata |
|
| 701 | 1 |
_aTetley _bT. D. _gTeresa |
|
| 701 | 1 |
_aChung _bK. F. _gKian Fan |
|
| 701 | 1 |
_aChen _bS. _gShu |
|
| 701 | 1 |
_aRyan _bM. P. _gMary |
|
| 701 | 1 |
_aPorter _bA. E. _gAlexandra |
|
| 701 | 1 |
_aAtochina _bE. N. _cbiophysicist _cDirector of RASA Center in Tomsk of Tomsk Polytechnic University _f1963- _gElena Nikolaevna _2stltpush _3(RuTPU)RU\TPU\pers\37356 |
|
| 701 | 1 |
_aZhang _bJu. _gJunfeng |
|
| 701 | 1 |
_aSchwander _bS. K. _gStephan |
|
| 701 | 1 |
_aGow _bA. J. _gAndrew John |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИнженерная школа природных ресурсов _bОтделение химической инженерии _h8085 _2stltpush _3(RuTPU)RU\TPU\col\23513 |
| 801 | 2 |
_aRU _b63413507 _c20210416 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.3389/fphar.2018.00213 | |
| 942 | _cCF | ||