000 04315nlm1a2200517 4500
001 665041
005 20231030041950.0
035 _a(RuTPU)RU\TPU\network\36240
090 _a665041
100 _a20210624a2020 k y0engy50 ba
101 0 _aeng
102 _aUS
135 _adrcn ---uucaa
181 0 _ai
182 0 _ab
200 1 _aVoltammetric Method for Determination of Glutathione on a Gold-Carbon-Containing Electrode
_fA. S. Gashevskaya, E. V. Dorozhko, E. I. Korotkova [et al.]
203 _aText
_celectronic
300 _aTitle screen
320 _a[References: 42 tit.]
330 _aGlutathione (GSH) is one of the most important thiol-containing antioxidants involved in various biochemical processes in the human body. Glutathione determination in biological fluids (saliva, urine, serum) and pharmaceutical preparations is rather important for clinical practice. Various analytical methods such as spectrophotometry, fluorimetry, high-performance liquid chromatography, NMR spectroscopy, capillary electrophoresis, and electrochemical methods are widely used for this purpose. Electrochemical methods are characterized by easy implementation, low cost, and possibility of miniaturization. The electrochemical behavior of reduced (GSH) and oxidized (GSSG) glutathione on a gold-carbon-containing electrode (AuCE) was studied using cathodic voltammetry with different methods of removing oxygen from an electrochemical cell: nitrogen sparging and addition of sodium sulfite (4 mol/dm3). It has been shown that traces of H2O2 that remain in the near-electrode layer on the AuCE even after oxygen removal influence the electrochemical properties of GSH at a cathode sweep of the potential from 0 to –1.8 V: GSH is oxidized by H2O2 to GSSG; the most important product of this reaction is O2. An indirect determination of GSH by the current of oxygen reduction in the Na2SO3 medium in the concentration range from 0.5 Ч 10–8 to 4.2 Ч 10–8 mol/dmm3 with a detection limit of 2.5 Ч 10–9 mol/dm3 is proposed. The developed voltammetric method is approved for the determination of GSH in certain pharmaceutical preparations.
333 _aРежим доступа: по договору с организацией-держателем ресурса
461 _tInorganic Materials
463 _tVol. 56, iss. 11
_v[P. 1362-1368]
_d2020
610 1 _aэлектронный ресурс
610 1 _aтруды учёных ТПУ
610 1 _aglutathione
610 1 _avoltammetry
610 1 _agold-carbon-containing electrode
610 1 _asodium sulfite
610 1 _athiol compounds
610 1 _aглутатионы
610 1 _aвольтамперометрия
610 1 _aсульфиты
610 1 _aтиоловые соединения
701 1 _aGashevskaya
_bA. S.
_gAnna Sergeevna
701 1 _aDorozhko
_bE. V.
_cchemist
_cEngineer of Tomsk Polytechnic University
_f1979-
_gElena Vladimirovna
_2stltpush
_3(RuTPU)RU\TPU\pers\32174
701 1 _aKorotkova
_bE. I.
_cchemist
_cProfessor of Tomsk Polytechnic University, Doctor of chemical science
_f1965-
_gElena Ivanovna
_2stltpush
_3(RuTPU)RU\TPU\pers\31278
701 1 _aPashkovskaya
_bE. A.
_gElvira Aleksandrovna
701 1 _aVoronova
_bO. A.
_cchemist
_cAssistant of Tomsk Polytechnic University, Candidate of chemical sciences
_f1979-
_gOlesya Aleksandrovna
_2stltpush
_3(RuTPU)RU\TPU\pers\32169
701 1 _aPlotnikov
_bE. V.
_cchemist
_cEngineer of Tomsk Polytechnic University
_f1983-
_gEvgeny Vladimirovich
_2stltpush
_3(RuTPU)RU\TPU\pers\32469
701 1 _aDerina
_bK. V.
_cchemist
_cAssociate Professor of Tomsk Polytechnic University, Candidate of chemical sciences
_f1989-
_gKseniya Vladimirovna
_2stltpush
_3(RuTPU)RU\TPU\pers\33919
701 1 _aLipskikh
_bO. I.
_cchemist
_cengineer of Tomsk Polytechnic University
_f1984-
_gOlga Ivanovna
_2stltpush
_3(RuTPU)RU\TPU\pers\38489
712 0 2 _aНациональный исследовательский Томский политехнический университет
_bИнженерная школа природных ресурсов
_bОтделение химической инженерии
_h8085
_2stltpush
_3(RuTPU)RU\TPU\col\23513
801 2 _aRU
_b63413507
_c20210624
_gRCR
856 4 _uhttps://doi.org/10.1134/S0020168520140071
942 _cCF