| 000 | 03897nlm1a2200577 4500 | ||
|---|---|---|---|
| 001 | 667038 | ||
| 005 | 20231030042057.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\38242 | ||
| 035 | _aRU\TPU\network\37940 | ||
| 090 | _a667038 | ||
| 100 | _a20220217a2018 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aCH | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aRough Titanium Oxide Coating Prepared by Micro-Arc Oxidation Causes Down-Regulation of hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor Jurkat T Cells _fI. A. Khlusov, L. S. Litvinova, V. V. Shupletsova [et al.] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: 57 tit.] | ||
| 330 | _aThe response of the human Jurkat T cell leukemia-derived cell line (Jurkat T cells) after 24 h of in vitro exposure to a titanium substrate (12•12•1 mm[mu]) with a bilateral rough (Ra=2.2-3.7 [mu]m) titanium oxide coating (rTOC) applied using the micro-arc method in a 20% orthophosphoric acid solution was studied. A 1.5-fold down-regulation of hTERT mRNA expression and decreases in CD3, CD4, CD8, and CD95 presentation and IL-4 and TNF[alpha] secretion were observed. Jurkat T cell inactivation was not correlated with the generation of intracellular reactive oxygen species (ROS) and was not mediated by TiO? nanoparticles with a diameter of 14 ± 8 nm at doses of 1 mg/L or 10 mg/L. The inhibitory effect of the rTOC (Ra=2.2-3.7 [mu]m) on the survival of Jurkat T cells (Spearman's coefficient rs=-0.95; n=9; p<0.0001) was demonstrated by an increase in the necrotic cell count among the cell population. In turn, an elevation of the Ra index of the rTOC was accompanied by a linear increase (r=0.6; p<0.000001, n=60) in the magnitude of the negative electrostatic potential of the titanium oxide surface. Thus, the roughness of the rTOC induces an electrostatic potential and decreases the viability of the immortalized Jurkat T cells through mechanisms unrelated to ROS generation. This may be useful for replacement surgery applications of rough TiO2 implants in cancer patients. | ||
| 461 | _tMaterials | ||
| 463 |
_tVol. 11, iss. 3 _v[360, 18 p.] _d2018 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aTiO2 nanoparticles | |
| 610 | 1 | _ain vitro | |
| 610 | 1 | _areactive oxygen species | |
| 610 | 1 | _asurface electrostatic potential | |
| 610 | 1 | _atitanium substrate | |
| 610 | 1 | _atumor cell death | |
| 610 | 1 | _aнаночастицы | |
| 610 | 1 | _aактивные формы | |
| 610 | 1 | _aэлектростатические потенциалы | |
| 610 | 1 | _aтитановые подложки | |
| 610 | 1 | _aопухолевые клетки | |
| 610 | 1 | _aкислород | |
| 701 | 1 |
_aKhlusov _bI. A. _cbiophysicist _cProfessor of Tomsk Polytechnic University, doctor of medical Sciences _f1963- _gIgor Albertovich _2stltpush _3(RuTPU)RU\TPU\pers\34907 |
|
| 701 | 1 |
_aLitvinova _bL. S. |
|
| 701 | 1 |
_aShupletsova _bV. V. _gValeria |
|
| 701 | 1 |
_aKhaziakhmatova _bO. G. |
|
| 701 | 1 |
_aMelashchenko _bE. S. |
|
| 701 | 1 |
_aYurova _bK. A. |
|
| 701 | 1 |
_aLeitsin _bV. N. |
|
| 701 | 1 |
_aKhlusova _bM. Yu. _gMarina Yurjevna |
|
| 701 | 1 |
_aPichugin _bV. F. _gVladimir Fedorovich |
|
| 701 | 1 |
_aSharkeev _bYu. P. _cphysicist _cProfessor of Tomsk Polytechnic University, Doctor of physical and mathematical sciences _f1950- _gYury Petrovich _2stltpush _3(RuTPU)RU\TPU\pers\32228 |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20220217 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.3390/ma11030360 | |
| 942 | _cCF | ||