| 000 | 03780nlm1a2200421 4500 | ||
|---|---|---|---|
| 001 | 667214 | ||
| 005 | 20231030042103.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\38419 | ||
| 035 | _aRU\TPU\network\27092 | ||
| 090 | _a667214 | ||
| 100 | _a20220304a2018 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aBranched poly (lactic acid) microparticles for enhancing the 5-aminolevulinic acid phototoxicity _fA. Di Martino, M. E. Trusova, P. S. Postnikov, V. Sedlarik |
|
| 203 |
_aText _celectronic |
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| 300 | _aTitle screen | ||
| 320 | _a[References: 31 tit.] | ||
| 330 | _aAn innovative microcarrier based on a carboxy-enriched and branched polylactic acid derivative was developed to enhance the in vitro phototoxicity of the photosensitizer and prodrug 5-aminolevulinic. Microparticles, prepared by double emulsion technique and loaded with the prodrug were carefully characterized and the effect of the polymer structure on the chemical, physical and biological properties of the final product was evaluated. Results showed that microparticles have a spherical shape and ability to allocate up to 30 ?g of the photosensitizer per mg of carrier despite their difference in solubility. Release studies performed in various simulated physiological conditions demonstrate the influence of the branched structure and the presence of the additional carboxylic groups on the release rate and the possibility to modulate it. In vitro assays conducted on human epithelial adenocarcinoma cells proved the not cytotoxicity of the carriers in a wide range of concentrations. The hemocompatibility and surface proteins adsorption were evaluated at different microparticles concentrations to evaluate the safety and estimate the possible microparticles residential time in the bloodstream. The advantages, of loading 5-aminolevulinic acid in the prepared carrier has been deeply described in terms of enhanced phototoxicity, compared to the free 5-aminolevulinic acid formulation after irradiation with light at 635 nm. The obtained results demonstrate the advantages of the prepared derivative compared to the linear polylactide for future application in photodynamic therapy based on the photosensitizer 5-aminolevulinic acid. | ||
| 333 | _aРежим доступа: по договору с организацией-держателем ресурса | ||
| 461 | _tJournal of Photochemistry and Photobiology B: Biology | ||
| 463 |
_tVol. 181 _v[P. 80-88] _d2018 |
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| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aphotodynamic therapy | |
| 610 | 1 | _amicroparticles | |
| 610 | 1 | _a5-Aminolevulinic acid | |
| 610 | 1 | _apolylactic acid | |
| 610 | 1 | _adrug delivery | |
| 701 | 1 |
_aDi Martino _bA. _corganic chemist _cresearch of Tomsk Polytechnic University _f1984- _gAntonio _2stltpush _3(RuTPU)RU\TPU\pers\39440 |
|
| 701 | 1 |
_aTrusova _bM. E. _corganic chemist _cAssociate professor of Tomsk Polytechnic University, Candidate of chemical sciences _f1982- _gMarina Evgenievna _2stltpush _3(RuTPU)RU\TPU\pers\31823 |
|
| 701 | 1 |
_aPostnikov _bP. S. _corganic chemist _cAssociate Professor of Tomsk Polytechnic University, Candidate of chemical sciences _f1984- _gPavel Sergeevich _2stltpush _3(RuTPU)RU\TPU\pers\31287 |
|
| 701 | 1 |
_aSedlarik _bV. _gVladimir |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20220304 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.1016/j.jphotobiol.2018.03.001 | |
| 942 | _cCF | ||