| 000 | 02839nlm1a2200493 4500 | ||
|---|---|---|---|
| 001 | 667254 | ||
| 005 | 20231030042104.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\38459 | ||
| 035 | _aRU\TPU\network\38358 | ||
| 090 | _a667254 | ||
| 100 | _a20220310a2018 k y0engy50 ba | ||
| 101 | 1 | _aeng | |
| 102 | _aNL | ||
| 135 | _aarcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aProduction of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli _fD. O. Ashcheulova, L. V. Efimova, A. Y. Lushchyk [et. al] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: 22 tit.] | ||
| 330 | _aRadiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand. | ||
| 461 | _tProtein Expression and Purification | ||
| 463 |
_tVol. 143 _v[P. 38-44] _d2018 |
||
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aantimicrobial peptides | |
| 610 | 1 | _aubiquicidine | |
| 610 | 1 | _aketosteroid isomerase | |
| 610 | 1 | _asize exclusion chromatography | |
| 610 | 1 | _aInclusion bodies | |
| 610 | 1 | _aкомпоненты | |
| 610 | 1 | _aантимикробные пептиды | |
| 610 | 1 | _aдиагностические системы | |
| 610 | 1 | _aэксклюзионная хроматография | |
| 701 | 1 |
_aAshcheulova _bD. O. |
|
| 701 | 1 |
_aEfimova _bL. V. _gLina Viktorovna |
|
| 701 | 1 |
_aLushchyk _bA. Y. |
|
| 701 | 1 |
_aYantsevich _bA. V. |
|
| 701 | 1 |
_aBaykov _bA. N. _gAleksandr Nikolaevich |
|
| 701 | 1 |
_aPershina _bA. G. _cbiologist _cAssociate Professor of Tomsk Polytechnic University, Candidate of biological sciences _f1981- _gAleksandra Gennadievna _2stltpush _3(RuTPU)RU\TPU\pers\32466 |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20220310 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.1016/j.pep.2017.10.011 | |
| 942 | _cCF | ||