| 000 | 03202nlm1a2200529 4500 | ||
|---|---|---|---|
| 001 | 667257 | ||
| 005 | 20231030042105.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\38462 | ||
| 090 | _a667257 | ||
| 100 | _a20220311a2021 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aDevelopment of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood _fA. Shabalina, D. Sharko, Yu. Glazyrin [et al.] |
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| 203 |
_aText _celectronic |
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| 300 | _aTitle screen | ||
| 320 | _a[References: 67 tit.] | ||
| 330 | _aWe describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability. A new blocking agent, a thiolated oligonucleotide with an unrelated sequence, was applied to fill the aptamer layer’s defects. Electrochemical aptasensor signal processing was enhanced using deep learning and computer simulation of the experimental data array. It was found that the combinations (coupled and tripled) of cyclic voltammogram features allowed for distinguishing between the samples from lung cancer patients and healthy candidates with a mean accuracy of 0.73. The capacitive component from the non-Faradic electrochemical impedance spectroscopy data indicated the tumor marker’s presence in a sample. These findings allowed for the creation of highly informative aptasensors for early lung cancer diagnostics. | ||
| 461 | _tSensors | ||
| 463 |
_tVol. 21, iss. 23 _v[7851, 20 p.] _d2021 |
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| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aelectrochemical aptasensor | |
| 610 | 1 | _abiosensing layer | |
| 610 | 1 | _auseful signal | |
| 610 | 1 | _alung cancer | |
| 610 | 1 | _ahuman blood | |
| 610 | 1 | _aбиосенсоры | |
| 701 | 1 |
_aShabalina _bA. _gAnastasiia |
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| 701 | 1 |
_aSharko _bD. _gDarya |
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| 701 | 1 |
_aGlazyrin _bYu. _gYury |
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| 701 | 1 |
_aBolshevich _bE. _gElena |
|
| 701 | 1 |
_aDubinina _bO. V. _cspecialist in the field of material science _cAssociate Professor of Tomsk Polytechnic University, Candidate of chemical sciences _f1984- _gOksana Valerievna _2stltpush _3(RuTPU)RU\TPU\pers\46874 |
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| 701 | 1 |
_aKim _bA. _gAnastasiia |
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| 701 | 1 |
_aVeprintsev _bD. _gDmitry |
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| 701 | 1 |
_aLapin _bI. _gIvan |
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| 701 | 1 |
_aZamay _bG. _gGalina |
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| 701 | 1 |
_aKrat _bA. _gAlexey |
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| 701 | 1 |
_aZamay _bS. _gSergey |
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| 701 | 1 |
_aSvetlichnyi _bV. _gValery |
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| 701 | 1 |
_aKichkailo _bA. _gAnna |
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| 701 | 1 |
_aBerezovski _bM. _gMaxim |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИнженерная школа новых производственных технологий _bОтделение материаловедения _h7871 _2stltpush _3(RuTPU)RU\TPU\col\23508 |
| 801 | 2 |
_aRU _b63413507 _c20220311 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.3390/s21237851 | |
| 942 | _cCF | ||