| 000 | 03784nlm1a2200577 4500 | ||
|---|---|---|---|
| 001 | 667961 | ||
| 005 | 20231030042132.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\39172 | ||
| 035 | _aRU\TPU\network\39146 | ||
| 090 | _a667961 | ||
| 100 | _a20220518a2021 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aCH | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aToxicological Analysis of Hepatocytes Using FLIM Technique: In Vitro versus Ex Vivo Models _fS. Rodimova, V. Elagin, M. Karabut [et al.] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: 85 tit.] | ||
| 330 | _aThe search for new criteria indicating acute or chronic pathological processes resulting from exposure to toxic agents, testing of drugs for potential hepatotoxicity, and fundamental study of the mechanisms of hepatotoxicity at a molecular level still represents a challenging issue that requires the selection of adequate research models and tools. Microfluidic chips (MFCs) offer a promising in vitro model for express analysis and are easy to implement. However, to obtain comprehensive information, more complex models are needed. A fundamentally new label-free approach for studying liver pathology is fluorescence-lifetime imaging microscopy (FLIM). We obtained FLIM data on both the free and bound forms of NAD(P)H, which is associated with different metabolic pathways. In clinical cases, liver pathology resulting from overdoses is most often as a result of acetaminophen (APAP) or alcohol (ethanol). Therefore, we have studied and compared the metabolic state of hepatocytes in various experimental models of APAP and ethanol hepatotoxicity. We have determined the potential diagnostic criteria including the pathologically altered metabolism of the hepatocytes in the early stages of toxic damage, including pronounced changes in the contribution from the bound form of NAD(P)H. In contrast to the MFCs, the changes in the metabolic state of hepatocytes in the ex vivo models are, to a greater extent, associated with compensatory processes. Thus, MFCs in combination with FLIM can be applied as an effective tool set for the express modeling and diagnosis of hepatotoxicity in clinics. | ||
| 338 |
_bРоссийский научный фонд _d19-15-00263 |
||
| 461 | _tCells | ||
| 463 |
_tVol. 10, iss. 11 _v[2894, 19 p.] _d2021 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aFLIM | |
| 610 | 1 | _amicrofluidic chip | |
| 610 | 1 | _ahepatocyte | |
| 610 | 1 | _aliver pathology | |
| 610 | 1 | _ametabolism | |
| 610 | 1 | _aчипы | |
| 610 | 1 | _aгепатоциты | |
| 610 | 1 | _aпаталогии | |
| 610 | 1 | _aпечень | |
| 610 | 1 | _aметаболизм | |
| 610 | 1 | _aтоксикологический анализ | |
| 701 | 1 |
_aRodimova _bS. _gSvetlana |
|
| 701 | 1 |
_aElagin _bV. _gVadim |
|
| 701 | 1 |
_aKarabut _bM. _gMariya |
|
| 701 | 1 |
_aKoryakina _bI. _gIrina |
|
| 701 | 1 |
_aTimin _bA. S. _cChemist _cAssociate Scientist of Tomsk Polytechnic University _f1989- _gAleksandr Sergeevich _2stltpush _3(RuTPU)RU\TPU\pers\37036 |
|
| 701 | 1 |
_aZagaynov _bV. _gVladimir |
|
| 701 | 1 |
_aZyuzin _bM. V. _gMikhail |
|
| 701 | 1 |
_aZagaynova _bE. V. _gElena Vladimirovna |
|
| 701 | 1 |
_aKuznetsova _bD. _gDarjya |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20220819 _gRCR |
|
| 856 | 4 | _uhttp://earchive.tpu.ru/handle/11683/72792 | |
| 856 | 4 | _uhttps://doi.org/10.3390/cells10112894 | |
| 942 | _cCF | ||