| 000 | 03466nlm1a2200481 4500 | ||
|---|---|---|---|
| 001 | 668681 | ||
| 005 | 20231030042156.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\39918 | ||
| 035 | _aRU\TPU\network\39841 | ||
| 090 | _a668681 | ||
| 100 | _a20230117a2022 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aCH | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aHow to Get More Silver? Culture Media Adjustment Targeting Surge of Silver Nanoparticle Penetration in Apricot Tissue during in Vitro Micropropagation _fC. Perez-Caselles, N. Alburquerque, L. Faize [et al.] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: 40 tit.] | ||
| 330 | _aThe use of silver nanoparticles (AgNPs) is increasing nowadays due to their applications against phytopathogens. Temporary Immersion Systems (TIS) allow the micropropagation of plants in liquid media. This work aims to develop an effective protocol for apricot micropropagation in TIS and to study the necessary conditions to introduce AgNPs in apricot plants, as well as the effect of its application on proliferation-related parameters. AgNPs were introduced in different media at a concentration of 100 mg L−1 to test the incorporation of silver to plant tissues. Silver content analysis was made by Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). The effect of initial shoot density and the addition of AgNPs on micropropagation were evaluated after four weeks in culture on TIS. Productivity, proliferation, shoot-length and leave surface were measured. The better micropropagation rate was obtained with 40 initial shoots, 2 min of immersion every 6 h and 3 min of aeration every 3 h. To introduce AgNPs in apricot plants it is necessary to culture them in liquid media without chloride in its composition. These results will contribute to the development of an in vitro protocol for virus inhibition by AgNPs application. This depends on the introduction of Ag nanoparticles within the plant tissues, and this is not possible if AgNPs after interaction with Cl- ions precipitate as silver chloride salts. | ||
| 461 | _tHorticulturae | ||
| 463 |
_tVol. 8, iss. 10 _v[885, 12 p.] _d2022 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aTIS | |
| 610 | 1 | _aAgNPs | |
| 610 | 1 | _aliquid media | |
| 610 | 1 | _avirucide | |
| 610 | 1 | _amicropropagation | |
| 610 | 1 | _aжидкие среды | |
| 701 | 1 |
_aPerez-Caselles _bC. _gCristian |
|
| 701 | 1 |
_aAlburquerque _bN. _gNuria |
|
| 701 | 1 |
_aFaize _bL. _gLydia |
|
| 701 | 1 |
_aBogdanchikova _bN. _gNina |
|
| 701 | 1 |
_aGarcia-Ramos _bJ. C. _gJuan Carlos |
|
| 701 | 1 |
_aRodriguez-Hernandez _bA. G. _gAna |
|
| 701 | 1 |
_aPestryakov _bA. N. _cChemist _cProfessor of Tomsk Polytechnic University, Doctor of Chemical Science _f1963- _gAleksey Nikolaevich _2stltpush _3(RuTPU)RU\TPU\pers\30471 |
|
| 701 | 1 |
_aBurgos _bL. _gLorenzo |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _bЛаборатория "Химическая инженерия и молекулярный дизайн" _h9100 _2stltpush _3(RuTPU)RU\TPU\col\27978 |
| 801 | 2 |
_aRU _b63413507 _c20230117 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.3390/horticulturae8100855 | |
| 942 | _cCF | ||