| 000 | 03616nlm1a2200553 4500 | ||
|---|---|---|---|
| 001 | 668884 | ||
| 005 | 20231030042203.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\40121 | ||
| 035 | _aRU\TPU\network\33047 | ||
| 090 | _a668884 | ||
| 100 | _a20230131a2022 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 102 | _aUS | ||
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aChronic toxicity of shrimp feed added with silver nanoparticles (Argovit-4®) in Litopenaeus vannamei and immune response to white spot syndrome virus infection _fC. R. Romo-Quinonez, P. Alvarez-Ruiz, C. H. Mejia-Ruiz [et al.] |
|
| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 330 | _aIn recent years, the application of silver nanoparticles (AgNPs) as antibacterial compounds has been widely used in human and veterinary medicine. In this work, we investigated the effects of AgNPs (Argovit-4®) as feed additives (feed-AgNPs) on shrimp (Litopenaeus vannamei) using three different methods: 1) chronic toxicity after 28 days of feeding, 2) Effects against white spot syndrome virus (WSSV) challenged by oral route, and 3) transcriptional responses of immune-related genes (PAP, ProPO, CTL-3, Crustin, PEN3, and PEN4) following WSSV infection. The results showed that the feed-AgNPs did not interfere with the growth and survival of shrimp. Also, mild lesions in the hepatopancreas were recorded, proportional to the frequency of the feed-AgNP supply. Challenge test versus WSSV showed that feeding every 7 days with feed-AgNPs reduced mortality, reaching a survival rate of 53%, compared to the survival rates observed in groups fed every 4 days, daily and control groups of feed-AgNPs for the 30%, 10%, and 7% groups, respectively. Feed-AgNPs negatively regulated the expression of PAP, ProPO, and Crustin genes after 28 days of treatment and altered the transcriptional responses of PAP, ProPO, CTL-3, and Crustin after WSSV exposure. The results showed that weekly feeding-AgNPs could partially prevent WSSV infection in shrimp culture. However, whether or not transcriptional responses against pathogens are advantageous remains to be elucidated. | ||
| 461 | _tPeerJ | ||
| 463 |
_tVol. 10 _v[e14231, 22 p.] _d2022 |
||
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _asilver nanoparticles | |
| 610 | 1 | _ashrimp | |
| 610 | 1 | _aaquaculture | |
| 610 | 1 | _achronic toxicity | |
| 610 | 1 | _aWSSV | |
| 610 | 1 | _aAgNP | |
| 610 | 1 | _aargovit | |
| 610 | 1 | _alitopenaeus vannamei | |
| 610 | 1 | _asilver fed | |
| 610 | 1 | _awhite spot syndrome virus | |
| 610 | 1 | _aнаночастицы | |
| 610 | 1 | _aинтоксикации | |
| 701 | 1 |
_aRomo-Quinonez _bC. R. _gCarlos |
|
| 701 | 1 |
_aAlvarez-Ruiz _bP. _gPindaro |
|
| 701 | 1 |
_aMejia-Ruiz _bC. H. |
|
| 701 | 1 |
_aBogdanchikova _bN. _gNina |
|
| 701 | 1 |
_aPestryakov _bA. N. _cChemist _cProfessor of Tomsk Polytechnic University, Doctor of Chemical Science _f1963- _gAleksey Nikolaevich _2stltpush _3(RuTPU)RU\TPU\pers\30471 |
|
| 701 | 1 |
_aGamez-Jimenez _bC. _gCarina |
|
| 701 | 1 |
_aValenzuela-Quinonez _bW. _gWenceslao |
|
| 701 | 1 |
_aMontoya-Mejia _bM. _gMagnolia |
|
| 701 | 1 |
_aPerez _bE. N. _gEusebio Nava |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20230131 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.7717/peerj.14231 | |
| 942 | _cCF | ||