| 000 | 05251nlm1a2200493 4500 | ||
|---|---|---|---|
| 001 | 669323 | ||
| 005 | 20231030042218.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\40563 | ||
| 035 | _aRU\TPU\network\40241 | ||
| 090 | _a669323 | ||
| 100 | _a20230322a2023 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aMicrocapsule-Based Dose-Dependent Regulation of the Lifespan and Behavior of Adipose-Derived MSCs as a Cell-Mediated Delivery System: In Vitro Study _fI. A. Khlusov, K. A. Yurova, V. V. Shupletsova [et al.] |
|
| 203 |
_aText _celectronic |
||
| 320 | _a[References: 73 tit.] | ||
| 330 | _aThe development of “biohybrid” drug delivery systems (DDS) based on mesenchymal stem/stromal cells (MSCs) is an important focus of current biotechnology research, particularly in the areas of oncotheranostics, regenerative medicine, and tissue bioengineering. However, the behavior of MSCs at sites of inflammation and tumor growth is relevant to potential tumor transformation, immunosuppression, the inhibition or stimulation of tumor growth, metastasis, and angiogenesis. Therefore, the concept was formulated to control the lifespan of MSCs for a specific time sufficient for drug delivery to the target tissue by varying the number of internalized microcontainers. The current study addressed the time-dependent in vitro assessment of the viability, migration, and division of human adipose-derived MSCs (hAMSCs) as a function of the dose of internalized polyelectrolyte microcapsules prepared using a layer-by-layer technique. Polystyrene sulfonate (PSS)—poly(allylamine hydrochloride) (PAH)-coated spherical micrometer-sized (diameter ~2–3 µm) vaterite (CaCO3) microcapsules (PAH-PSS)6 with the capping PSS layer were prepared after dissolution of the CaCO3 core template. The Cell-IQ phase contrast imaging results showed that hAMSCs internalized all (PAH-PSS)6 microcapsules saturating the intercellular medium (5–90 particles per cell). A strong (r > 0.7) linear dose-dependent and time-dependent (up to 8 days) regression was observed between the in vitro decrease in cell viability and the number of internalized microvesicles. The approximate time-to-complete-death of hAMSCs at different concentrations of microcapsules in culture was 428 h (1:5 ratio), 339 h (1:10), 252 h (1:20), 247 h (1:45), and 170 h (1:90 ratio). | ||
| 330 | _aBy varying the number of microcontainers loaded into the cells (from 1:10 to 1:90), a dose-dependent exponential decrease in both the movement rate and division rate of hAMSCs was observed. A real-time cell analysis (RTCA) of the effect of (PAH-PSS)6 microcapsules (from 1:5 to 1:20) on hAMSCs also showed a dose- and time-dependent decrease in cell longevity after a 50h study at ratios of 1:10 and 1:20. The incorporation of microcapsules (1:5, 1:20, and 1:45) resulted in a dose-dependent increase in 24-48 h secretion of GRO-a (CXCL1), MIF, and SDF-1a (CXCL12) chemokines in hAMSC culture. In turn, the normalization or inhibition of chemokine secretion occurred after 72 h, except for MIF levels below 5-20 microcapsules, which were internalized by MSCs. Thus, the proposed concept of controlling the lifespan of MSC-based DDS using a dose of internalized PAH-PSS microcapsules could be useful for biomedical applications. (PAH-PSS)6 microcapsule ratios of 1:5 and 1:10 have little effect on the lifespan of hAMSCs for a long time (up to 14-18 days), which can be recommended for regenerative therapy and tissue bioengineering associated with low oncological risk. The microcapsule ratios of 1:20 and 1:45 did not significantly restrict the migratory activity of hAMSCs-based DDS during the time interval required for tissue delivery (up to 4-5 days), followed by cell death after 10 days. Therefore, such doses of microcapsules can be used for hAMSC-based DDS in oncotheranostics. | ||
| 461 | _tInternational Journal of Molecular Sciences | ||
| 463 |
_tVol. 24, iss. 1 _v[292, 20 p. ] _d2023 |
||
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _ahuman adipose-derived MSCs | |
| 610 | 1 | _apolyelectrolyte microcapsules | |
| 610 | 1 | _alayer-by-layer technique | |
| 610 | 1 | _aphagocytosis | |
| 610 | 1 | _acell viability | |
| 610 | 1 | _amigration | |
| 610 | 1 | _adivision | |
| 701 | 1 |
_aKhlusov _bI. A. _cbiophysicist _cProfessor of Tomsk Polytechnic University, doctor of medical Sciences _f1963- _gIgor Albertovich _2stltpush _3(RuTPU)RU\TPU\pers\34907 |
|
| 701 | 1 |
_aYurova _bK. A. |
|
| 701 | 1 |
_aShupletsova _bV. V. _gValeria |
|
| 701 | 1 |
_aKhaziakhmatova _bO. _gOlga |
|
| 701 | 1 |
_aMalashchenko _bV. V. _gVladimir |
|
| 701 | 1 |
_aKudryavtseva _bV. L. |
|
| 701 | 1 |
_aKhlusova _bM. Yu. _gMarina |
|
| 701 | 1 |
_aSukhorukov _bG. _gGleb |
|
| 701 | 1 |
_aLitvinova _bL. _gLarisa |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20230322 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.3390/ijms24010292 | |
| 942 | _cCF | ||