| 000 | 03775nlm1a2200481 4500 | ||
|---|---|---|---|
| 001 | 669419 | ||
| 005 | 20231030042222.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\40659 | ||
| 035 | _aRU\TPU\network\34977 | ||
| 090 | _a669419 | ||
| 100 | _a20230504a2023 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aAre silver nanoparticles the “silver bullet” to promote diterpene production in Stevia rebaudiana? _fI. Andujar, N. Gonzalez, J. C. Garcia-Ramos [et al.] |
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| 203 |
_aText _celectronic |
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| 300 | _aTitle screen | ||
| 330 | _aS. rebaudiana is a sought after sweetener because of its low-calorie properties. However, the supply of suitable quantities of high quality propagation material is limited by inefficient propagation methods using conventional strategies. In vitro techniques combined with nanotechnology tools offer an attractive alternative not only for improved propagation but also for the stimulation of secondary metabolites which represent the targeted sweetener product for this crop. This report provides an evaluation of silver nanoparticles applied in temporary immersion bioreactors for the abovementioned purpose. Different levels of AgNPs were supplied (0.0—37.5 mg/L) and after 21 d of growth, morphological and biochemical indicators were evaluated. Silver nanoparticles at 25 and 37.5 mg/L decreased shoot multiplication rate, shoot length, and the number of nodes and leaves per shoot compared with the control while no adverse effect was found at the lower tested concentration (12.5 mg/L). Shoot fresh and dry weights also showed statistically significant differences. Regarding the biochemical phenotypes, chlorophyll a, carotenoids and soluble phenolics were increased in plants supplied with 25 mg/L AgNPs, with the latter two indicators suggesting oxidative stress. Interestingly, endogenous levels of diterpenes were significantly increased with the application of 12.5 mg/L AgNPs. It is suggested that AgNPs show potential to act as elicitors to promote the production of diterpenes in stevia but that further work is required to understand the balance between oxidative damage and secondary metabolite production and that optimization of the protocol is required to improve the propagation potential of this strategy. | ||
| 333 | _aРежим доступа: по договору с организацией-держателем ресурса | ||
| 461 | _tPlant Cell, Tissue and Organ Culture (PCTOC) | ||
| 463 |
_tVol. XX, iss. XX _v[7 p.] _d2023 |
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| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _ain vitro culture systems | |
| 610 | 1 | _ananoparticle | |
| 610 | 1 | _aplant natural products | |
| 610 | 1 | _asteviol glycosides | |
| 610 | 1 | _asweet grass | |
| 610 | 1 | _aнаночастицы | |
| 610 | 1 | _aгликозиды | |
| 701 | 1 |
_aAndujar _bI. _gIvan |
|
| 701 | 1 |
_aGonzalez _bN. _gNoel |
|
| 701 | 1 |
_aGarcia-Ramos _bJ. C. _gJuan Carlos |
|
| 701 | 1 |
_aBogdanchikova _bN. _gNina |
|
| 701 | 1 |
_aPestryakov _bA. N. _cChemist _cProfessor of Tomsk Polytechnic University, Doctor of Chemical Science _f1963- _gAleksey Nikolaevich _2stltpush _3(RuTPU)RU\TPU\pers\30471 |
|
| 701 | 1 |
_aConcepcion _bO. _gOscar |
|
| 701 | 1 |
_aLorenzo _bJ. C. _gJose Carlos |
|
| 701 | 1 |
_aEscalona _bM. _gMaritza |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20230504 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.1007/s11240-023-02450-5 | |
| 942 | _cCF | ||