| 000 | 03464nlm1a2200505 4500 | ||
|---|---|---|---|
| 001 | 669441 | ||
| 005 | 20231030042223.0 | ||
| 035 | _a(RuTPU)RU\TPU\network\40681 | ||
| 035 | _aRU\TPU\network\40273 | ||
| 090 | _a669441 | ||
| 100 | _a20230510a2023 k y0engy50 ba | ||
| 101 | 0 | _aeng | |
| 135 | _adrcn ---uucaa | ||
| 181 | 0 | _ai | |
| 182 | 0 | _ab | |
| 200 | 1 |
_aPeptide ligands on the PEGylated nanoparticle surface and human serum composition are key factors for the interaction between immune cells and nanoparticles _fA. G. Pershina, A. M. Demin, N. A. Perekucha [et al.] |
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| 203 |
_aText _celectronic |
||
| 300 | _aTitle screen | ||
| 320 | _a[References: 60 tit.] | ||
| 330 | _aThe architecture of a nanoparticles’ surface formed due to a modification with a ligand and protein corona formation in biofluids is critical for interactions with cells in vivo. Here we studied interactions of immune cells with magnetic nanoparticles (MNPs) covalently modified with polyethylene glycol (PEG) and their counterparts conjugated with peptides: a pH (low) insertion peptide (pHLIP) and cycloRGD as a targeting ligand in human serum. The conjugation of MNPs-PEG with pHLIP, but not with cycloRGD, enhanced the association of these particles with mononuclear phagocytic cells in vitro and in vivo. We did not find a clear difference in protein corona composition between the pHLIP-modified and parental PEGylated nanoparticles. Analysis of the effect of autologous human serum on MNP uptake by monocytes showed that the efficiency of endocytosis varies among healthy donors and depends on intrinsic properties of serum. Nevertheless, using classic blood, coagulation, biochemical tests, and anti-PEG IgG serum level, we failed to identify the cause of the observed interdonor variation. These individual differences should be taken into consideration during testing of nanotherapeutics. | ||
| 333 | _aРежим доступа: по договору с организацией-держателем ресурса | ||
| 461 | _tColloids and Surfaces B: Biointerfaces | ||
| 463 |
_tVol. 221 _v[112981, 13 p.] _d2023 |
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| 610 | 1 | _aэлектронный ресурс | |
| 610 | 1 | _aтруды учёных ТПУ | |
| 610 | 1 | _aimmune cells | |
| 701 | 1 |
_aPershina _bA. G. _cbiologist _cAssociate Professor of Tomsk Polytechnic University, Candidate of biological sciences _f1981- _gAleksandra Gennadievna _2stltpush _3(RuTPU)RU\TPU\pers\32466 |
|
| 701 | 1 |
_aDemin _bA. M. |
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| 701 | 1 |
_aPerekucha _bN. A. _gNataljya Andreevna |
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| 701 | 1 |
_aBrikunova _bO. Ya. _gOlga Yaroslavovna |
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| 701 | 1 |
_aEfimova _bL. V. _gLina Viktorovna |
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| 701 | 1 |
_aNevskaya _bK. V. |
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| 701 | 1 |
_aVakhrushev _bA. V. _gAleksandr |
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| 701 | 1 |
_aZgoda _bV. G. _gViktor |
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| 701 | 1 |
_aUymin _bM. A. _gMikhail Aleksandrovich |
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| 701 | 1 |
_aMinin _bA. S. _gArtyom |
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| 701 | 1 |
_aMalkeeva _bD. _gDina |
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| 701 | 1 |
_aKiseleva _bE. _gElena |
|
| 701 | 1 |
_aZima _bA. P. _gAnastasiya Pavlovna |
|
| 701 | 1 |
_aKrasnov _bV. P. _gViktor |
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| 701 | 1 |
_aOgorodova _bL. M. _gLyudmila Mikhaylovna |
|
| 712 | 0 | 2 |
_aНациональный исследовательский Томский политехнический университет _bИсследовательская школа химических и биомедицинских технологий _c(2017- ) _h8120 _2stltpush _3(RuTPU)RU\TPU\col\23537 |
| 801 | 2 |
_aRU _b63413507 _c20230510 _gRCR |
|
| 856 | 4 | _uhttps://doi.org/10.1016/j.colsurfb.2022.112981 | |
| 942 | _cCF | ||